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| Registros recuperados: 17 | |
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| Iorio,Mario; Vincenti,Donatella; Annunziata,Mario; Rullo,Rosario; Bonamassa,Raffaele; Di Luccia,Aldo; Pieragostini,Elisa. |
| On 398 river buffalo samples, randomly collected in distinct breeding areas of the Campania region, high-resolution analytical systems were used to identify both qualitative and quantitative variations of the Hb phenotype. Polyacrylamide gel isoelectric focusing and HPLC were used to determine the ratio between HBA1 and HBA2 globin chains; restriction endonuclease analysis was performed to assess whether quantitative variations in Hb bands were related to an unusual number of a-globin genes. In the two buffalo subpopulations, allele frequencies of the alpha and beta globin systems were calculated, and F statistics (FIS, FIT and FST) were estimated as parameters of genetic diversity. The results suggest that: i) as shown by RFLP analysis, only a couple of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phenotype; RFLP; Globin genes; Haplotype; Gene frequencies. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200007 |
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| García Pérez, Florinda. |
| El psílido asiático de los cítricos, Diaphorina citri Kuwayama (Hemiptera: Liviidae), es una plaga de importancia cuarentenaria por ser un eficiente vector de la bacteria Candidatus Liberibacter asiaticus, agente causal del Huanglongbing (HLB). Al ser una plaga invasora y de reciente introducción a nuestro país, es posible que el aislamiento geográfico desde su lugar de origen derive en variaciones morfométricas y biológicas que dificulten la definición de una estrategia de manejo. Por lo anterior en este estudio se planteó realizar estudios de morfometría y conocer la diversidad genética de D. citri mediante el uso de una región del marcador genético mitocondrial Citocromo oxidasa subunidad I (COI) en psílidos adultos recolectados en hospedantes rutáceas... |
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Palavras-chave: Procesos genales; Variantes morfométricas; Haplotipo; Genal processes; Morphometric variants; Haplotype; Entomología y Acarología; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1983 |
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| Brandão,Paulo E.; Hora,Aline S.; Silva,Sheila O. S.; Taniwaki,Sueli A.; Berg,Mikael. |
| Abstract Avian coronavirus (AvCoV) is ubiquitously present on poultry as a multitude of virus lineages. Studies on AvCoV phenotypic traits are dependent on the isolation of field strains in chicken embryonated eggs, but the mutant spectrum on each isolate is not considered. This manuscript reports the previously unknown HTS (high throughput sequencing)-based complete genome haplotyping of AvCoV isolates after passages of two field strains in chicken embryonated eggs. For the first and third passages of strain 23/2013, virus loads were 6.699 log copies/ μL and 6 log copies/ μL and, for 38/2013, 5.699 log copies/μL and 2.699 log copies/μL of reaction, respectively. The first passage of strain 23/2013 contained no variant haplotype, while, for the third... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian coronavirus; Haplotype; Mutant spectrum; NGS; Evolution. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400202 |
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| Zhang,T; Du,W; Lu,H; Wang,L. |
| ABSTRACT The black-bone chicken has special economic value in Chinese poultry breeds, which also are valued for the medicinal properties of their meat in traditional Chinese medicine. In order to protect the genetic resources of native black-bone chicken breeds, we analyzed the genetic diversity and matrilineal components of 64 mtDNA D-loop partial sequences from three native black-bone chicken breeds, together with reported 596 black-bone chicken mtDNA sequences from China, Japan, and Korea. A total of 108 haplotypes were observed from 73 variable sites. These domestic chicken mtDNA sequences could be assigned into seven clades (A-G). The results indicated that 71.97% of the black-bone haplotypes were related to the reference sequence that may originate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chinese Black-bone Chicken; MtDNA; D-loop region; Haplotype; Maternal origin. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300565 |
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| Yang,Xiuqin; Li,Haitao; Guan,Qingzhi; Liu,Di. |
| The Toll-like receptor 5 (TLR5) recognizes flagellin of Gram-positive and -negative bacteria and plays an important role in the host defense system. Here, we surveyed single nucleotide polymorphisms (SNPs) in the coding sequence of the porcine TLR5 gene in 83 individuals from five pig breeds, these including Chinese local populations and Western commercial pig breeds. A total of 19 medium polymorphic SNPs (0.25 < PIC < 0.5) were identified, three of which were missense mutations that clustered within the extracellular domain of TLR5. One of the non-synonymous SNPs fell within a 228-amino acid region which has been shown to be important for flagellin recognition. Four SNPs were only found with high frequencies in Oriental pig breeds. The 19 SNPs were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine; TLR5; SNPs; Haplotype. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100006 |
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| Jacobs,Clifford; Pearce,Brendon; Du Plessis,Mornè; Hoosain,Nisreen; Benjeddou,Mongi. |
| Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms in the gene coding for human organic cation transporter 1, SLC22A1, are increasingly being recognized as a possible mechanism explaining the variable response to clinical drugs, which are substrates for this transporter. The genotypic and allelic distributions of 19 nonsynonymous and one intronic SLC22A1 single nucleotide polymorphisms were determined in 148 healthy Xhosa participants from South Africa, using a SNAPshot® multiplex assay. In addition, haplotype structure for SLC22A1 was inferred from the genotypic data. The minor allele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymorphism; Haplotype; Population genetic structure; Genotyping; Genetic variability. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300006 |
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| Zhang,Xiaoqing; Ao,Guokun; Wang,Yuewen; Yan,Wei; Wang,Min; Chen,Erfei; Yang,Fangfang; Yang,Jin. |
| In this report, we describe combined polymorphisms of the UGT1A9, UGT1A7 and UGT1A1 genes in 100 unrelated, healthy Chinese Han subjects. The functional regions of these genes were sequenced and comprehensively analyzed for genetic polymorphisms. Thirty variants were detected, including five novel forms. Tentative functional predictions indicated that a Cys → Arg substitution at position 277 in the UGT1A7 gene could alter the protein conformation and that 12460T > G in the 3'UTR might influence protein translation through specifically expressed miRNAs. UGT1A9*1b was a major functional variant in the subjects examined whereas the *1f allele had a frequency of only 0.5%. A special functional haplotype (GAGAAC) was identified for UGT1A9, 1A7 and 1A1. These... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chinese Han; Genetic polymorphisms; Haplotype; UGT1A. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300008 |
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| Fonseca,Ana Carolina Proença da; Marchesini,Bruna; Zembrzuski,Verônica Marques; Voigt,Danielle Dutra; Ramos,Vivianne Galante; Carneiro,João Regis Ivar; Nogueira Neto,José Firmino; Cabello,Giselda Maria Kalil de; Cabello,Pedro Hernán. |
| Abstract Obesity is a major public health problem worldwide. It has a complex etiology, influenced by environmental and genetic factors. FTO has been recognized as an important genetic factor for obesity development. This study evaluated the contribution of FTO polymorphisms (rs9939609 and rs17817449) for extreme obesity in terms of the period of obesity onset, anthropometric, and biochemical parameters. The haplotype and the combined effects of FTO risk alleles on obesity susceptibility were evaluated. We investigated 169 normal-weight subjects (body mass index, BMI: 22.8 [21.0; 24.0] kg/m2) and 123 extremely obese individuals (BMI: 47.6 [44.1; 53.1] kg/m2). Genotyping was performed by real time PCR. Our results showed a strong association between FTO... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BMI; Extreme obesity; FTO; Haplotype; Polymorphisms. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100111 |
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| Ferreira,Jéssica Rosset; Faria,Bruna Franciele; Comar Junior,Moacyr; Delatorre,Carla Andréa; Minella,Euclydes; Pereira,Jorge Fernando. |
| Abstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aluminium tolerance; Citrate transporter; Haplotype; Hordeum vulgare; Single nucleotide polymorphism. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480 |
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| Borba,Rafael Splendore de; Zawadzki,Claudio Henrique; Oliveira,Claudio; Perdices,Anabel; Parise-Maltempi,Patrícia Pasquali; Alves,Anderson Luis. |
| In this study, phylogenetic and phylogeographic analyses of populations identified as Hypostomus strigaticeps from the upper Paraná River basin were conducted in order to test whether these different populations comprises cryptic species or structured populations and to assess their genetic variability. The sequences of the mitochondrial DNA ATP sintetase (subunits 6/8) of 27 specimens from 10 populations (one from Mogi-Guaçu River, five from Paranapanema River, three from Tietê River and one from Peixe River) were analyzed. The phylogeographic analysis showed the existence of eight haplotypes (A-H), and despite the ancestral haplotype includes only individuals from the Tietê River basin, the distribution of H. strigaticeps was not restricted to this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ATPase; Bayesian Inference; Haplotype; Nested clade; Phylogeny. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100111 |
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| Yan,LJ; Fang,XT; Liu,Y; Zhang,CL; Chen,H. |
| ABSTRACT Animal lysozymes, which have been studied in many of invertebrate and vertebrate species, have been characterized and demonstrated to be immune-associated molecules, digestive enzymes and multifunctional molecules. The purpose of this study was to detect the connection between lysozyme-gene polymorphism and the production traits of a Chinese native chicken breed (Langshan chicken). Four single nucleotide mutation sites were identified: G345A, C1726T, G1836A, A1838G. By the linkage disequilibrium analysis, six haplotypes and 15haplotype combinations were depicted in the studied population. The statistical analysis demonstrated that the SNPs and the haplotype combinations are related to body weight at sixteen weeks of age in Langshan chickens... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lysozyme gene; Haplotype; Association; Langshan chicken; PCR-SSCP. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100053 |
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| Wang,ZhePeng; Meng,GuoHua; Li,Na; Yu,MingFen; Liang,XiaoWei; Min,YuNa; Liu,FuZhu; Gao,YuPeng. |
| Abstract The very low-density lipoprotein receptor (VLDLR) transports egg yolk precursors into oocytes. However, our knowledge of the distribution patterns of VLDLR variants among breeds and their relationship to egg production is still incomplete. In this study, eight single nucleotide polymorphisms (SNPs) that account for 87% of all VLDLR variants were genotyped in Nick Chick (NC, n=91), Lohmann Brown (LohB, n=50) and Lueyang (LY, n=381) chickens, the latter being an Chinese indigenous breed. Egg production by NC and LY chickens was recorded from 17 to 50 weeks. Only four similar haplotypes were found in NC and LohB, of which two accounted for 100% of all NC haplotypes and 92.5% of LohB haplotypes. In contrast, there was considerable haplotypic diversity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Egg production; Haplotype; Very low-density lipoprotein receptor (VLDLR). |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300380 |
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| Machado,Alessandro Lima; Meira,Ariana Nascimento; Jucá,Adriana de Farias; Azevedo,Hymerson Costa; Muniz,Evandro Neves; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista. |
| ABSTRACT: Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hormone; Carcass; Growth; Haplotype; Ovine. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000300401 |
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| Registros recuperados: 17 | |
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